Haoyue patented strain

Lactobacillus fermentum grx08

Patent number: ZL 2008 1 0023012.0

Regulates blood lipid levels Assists in lowering blood lipids

About blood lipids

The number of people dying from heart disease and cerebrovascular disease is as high as 17 million every year in the world, and the number of deaths from cardiovascular disease in the world accounts for about 30% of the total number of deaths each year, and it is expected to increase to 23 million by 2030. There are many risk factors for cardiovascular disease. Relevant studies have shown that abnormal blood lipid levels, especially the occurrence and development of total cholesterol diseases, are closely related. In recent decades, cardiovascular diseases have seriously threatened human health. Vascular diseases caused by abnormalities have taken the lead in death.

Blood lipids are essential substances for basic metabolism of living cells. Generally speaking, the main components of blood lipids are triglycerides, cholesterol, phospholipids, and free fatty acids. Among them, triglycerides are involved in energy metabolism in the human body, while cholesterol is mainly used for the synthesis of cell plasma membrane, steroid hormones and bile acids.

The dangers of dyslipidemia

The hyperlipidemia we often talk about should actually be called dyslipidemia. Among the blood lipid indicators, in addition to total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C), high blood lipid indicators are abnormal manifestations. The reduction of high-density lipoprotein cholesterol (HDL-C) is also one of the manifestations of blood lipid problems.

For healthy lipid levels in blood samples, it is better to have lower TC/TG/LDL-C levels, and higher HDL-C levels. Surveys and studies show that among adults in my country, more than 40% of adults with dyslipidemia are dyslipidemia. Hyperlipidemia is also a wealthy disease that people are very worried about now, and it can also cause a variety of complications.

A large number of research data show that hyperlipidemia is an independent and important risk factor for stroke, coronary heart disease, myocardial infarction and sudden cardiac death. In addition, hyperlipidemia is also an important risk factor for hypertension, impaired glucose tolerance, and diabetes. Hyperlipidemia can also lead to fatty liver, cirrhosis, cholelithiasis, pancreatitis, fundus hemorrhage, blindness, peripheral vascular disease, claudication, hyperuricemia, etc.

How to improve dyslipidemia is currently mostly treated with chemical synthetic drugs. Long-term use of patients will cause certain adverse reactions to the body. Therefore, improving blood lipid levels through non-drug approaches has attracted more and more attention. Recent studies have suggested that in addition to regulating the host's intestinal flora and enhancing immunity, probiotics can help regulate blood lipid metabolism to a certain extent.

The discovery journey of probiotics to lower blood lipids

How were probiotics discovered to have lipid lowering effects?

In 1996 the University of Belgrade (University of Belgrade) conducted a study on 17 non-insulin diabetic patients. The scientists administered 200 ml of fermented yogurt (>1 million CFU per ml of probiotics) three times a day for 12 weeks. In an index test 4 weeks later, the subjects' serum cholesterol levels were significantly reduced by 20% thanks to the probiotics.

The reasons for the analysis may be: some probiotics can absorb cholesterol and bind part of the cholesterol to the cell membrane or cell wall of bacteria; the bile salt hydrolase produced by some lactobacilli can dissociate the bound bile salt into free bile acid and form co-precipitation with cholesterol. In addition, free bile acids cannot be effectively reabsorbed by the intestinal lumen and excreted from the feces in the enterohepatic circulation, which is conducive to the formation of new bile acids from cholesterol in the blood and promotes the catabolism of cholesterol; the propionate produced by the fermentation of carbohydrates by probiotics It can inhibit the synthesis of cholesterol and fatty acids in the liver, thereby reducing the levels of total cholesterol and triglycerides in serum.

So can all probiotics help us lower blood lipids? The answer is NO. Among the many probiotics, only a few probiotics can lower blood lipids, eliminate cholesterol in the blood vessel wall, relieve atherosclerosis, fatty liver and other conditions.

grx08 product introduction

Lactobacillus fermentum grx08 (Lactobacillus fermentum grx08, L. fermentum grx08) is a probiotic with auxiliary hypolipidemic function screened in the intestines of Zirugao longevity population. It was screened by Shanghai Haoyue and Yangzhou University through joint research.

Lactobacillus fermentum grx08 is widely used in fermented dairy products and dietary supplements due to its high in vitro cholesterol-lowering ability, strong gastric juice, intestinal juice, bile salt tolerance, and strong intestinal adhesion ability. Then let's take a look at this patented strain-grx08, which is known as the "lipid-lowering master" in the Lactobacillus fermentum world.

In 2013, the preservation number of strains declared for preservation: CGMCC NO. 7695

In 2014, it obtained the national patent authorization patent number: ZL 2013 1 0309222.7

Patent name: Lactobacillus fermentum grx08 with auxiliary hypolipidemic function and its application

Transferred to Shanghai Haoyue in 2019

In vitro efficacy of grx08

How to make an excellent cholesterol-lowering probiotic strain, one of the important criteria is that it has a high cholesterol-lowering ability and its intestinal adhesion ability, and it should also have a good effect on gastric juice, intestinal juice and bile salts. tolerance. Lactobacillus fermentum grx08 has many characteristics such as high cholesterol-lowering ability in vitro, strong gastric acid and bile tolerance, and good persistence of intestinal colonization, and these characteristics constitute the basis of its use as a health-promoting and cholesterol-lowering strain.

The specific data are as follows:

1. In vitro cholesterol lowering ability: The cholesterol degradation rate of grx08 can reach 63.73%.

2. Gastric acid and bile tolerance: grx08 had higher survival rates at pH 3.0 and pH 2.0, 37.01% and 68.55%, respectively.

3. Intestinal colonization ability: grx08 cells have a high adhesion rate and can effectively colonize the intestinal tract and exert a probiotic effect.

In vivo efficacy properties of grx08

Under a variety of experimental studies, Lactobacillus fermentum grx08 has significant positive effects on regulating blood lipid levels, promoting the degradation and elimination of cholesterol in the liver and gallbladder, promoting the formation and excretion of bile acids, and observing heart and liver slices. (Please contact us for more valid experimental data)

Other related research on grx08

1. The research on the freeze-drying process of grx08 ensures that the lactic acid bacteria can not only ensure a high number of viable bacteria after freeze-drying, but also reduce the physiological damage to the lactic acid bacteria caused by freeze-drying, and ensure the vitality of the lactic acid bacteria.

2. Mixing grx08 with other probiotics can have a synergistic effect and improve the probiotic function of probiotics.

3. The mixed fermentation study of grx08 and Streptococcus thermophilus can obtain high-quality fermented milk with good water holding capacity, moderate viscosity, high acetaldehyde content and diacetyl content, and can ensure the stability of the product during the shelf life.

[1] Li Yan, Yang Jing, Huang Yujun. Effects of vacuum freeze-drying on the fermentation stability of lactic acid bacteria [J]. Modern Food, 2017(18):72-76.

[2] Yin Boxing. Study on the effect and mechanism of lactic acid bacteria fermented milk assisted in lowering blood lipids [D]. Yangzhou University, 2019.

[3] Yin Boxing, Wa Yunchao, Huang Yujun, Xu Guangxin, Gu Ruixia. Study on fermentation conditions of human-derived multi-strain probiotics [J]. China Dairy Industry, 2019, 47(03): 4-7+16.

[4] Lu Jiacheng. Effects of Streptococcus thermophilus on the quality of fermented milk of Lactobacillus fermentum grx08 [D]. Yangzhou University, 2019.

[5] Yin Boxing, Wa Yunchao, Huang Yujun, Xu Guangxin, Gu Ruixia. In vitro study on cholesterol lowering of different lactic acid bacteria strains and mixed strains [J]. Journal of Yangzhou University (Agriculture and Life Science Edition), 2020, 41(01) :44-49+61.

Food industry application of grx08

Lactobacillus fermentum grx08 powder

Viable count: 1×10^11 CFU/g,

Packing specification: 10D/bag, 1kg/bag, or customized

Storage conditions: Store at -18°C

Application of solid beverage products

Solid beverage and yogurt applications

The patented Lactobacillus fermentum grx08 can be used in beverages, yogurt, snacks, dietary fiber and other foods, and can impart more reliable functionality to the products.

At present, Lactobacillus fermentum grx08 has been successfully used in dietary supplements and dairy products.

At the same time we are opening more possibilities for Lactobacillus fermentum grx08.